Meno:Michaela
Priezvisko:©andalová
Názov:Computational Design of Probes for the Hyb-Seq Protocol
Vedúci:Mgr. Matúą Kempa
Rok:2018
Kµúčové slová:Hyb-seq, probes, phylogeny, genetic markers
Abstrakt:The Hyb-Seq protocol is na approach that enables finding effective genetic markers for study of phylogeny. We used an existing script – Sondovač – which is an automated pipeline that creates orthologous low-copy nuclear probes for use in plant phylogeny. Sondovač uses transcriptome and genome skim data and creates probes for later target enrichment. We created a number of initial probes from two initial sets of plant data. From these probes, we picked those we considered to be relevant genetic markers using a script we coded. The script helped to fulfill the requirements we placed on the probes and created probes that are made of 1, 000, 000 base pairs.

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dada_script.tar.gz

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